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Fluorescence Studies on Biological Membranes
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9781461393597

Details of the book - Fluorescence Studies on Biological Membranes


EAN (ISBN-13): 9781461393597
Publishing year: 2012
Publisher: Springer US

Book in our database since 2016-06-13T23:43:01+01:00 (London)
Detail page last modified on 2023-11-12T17:55:14+00:00 (London)
ISBN/EAN: 9781461393597

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978-1-4613-9359-7
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Book title: biological membranes

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Author: Herwig J. Hilderson
Title: Subcellular Biochemistry; Fluorescence Studies on Biological Membranes
Publisher: Springer; Springer US
465 Pages
Publishing year: 2012-12-06
New York; NY; US
Language: English
53,49 € (DE)
55,00 € (AT)
59,00 CHF (CH)
Available
465 p.

EA; E107; eBook; Nonbooks, PBS / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Biomembran; DNA; Lipid; Oxidation; Polypeptide; plasma membrane; proteins; reductase; C; Biochemistry; Animal Anatomy; Plant Science; Biomedical and Life Sciences; Zoologie und Tierwissenschaften; Botanik und Pflanzenwissenschaften; BC

1 Biomembrane Structure and Dynamics Viewed by Fluorescence.- 1. Introduction to Fluorescence.- 2. Dynamics and Structure of Membranes.- 3. Fluorescence Techniques and What They Make Visible.- 4. Summary and Conclusions.- 5. References.- 2 Dynamic Structure of Membranes and Subcellular Components Revealed by Optical Anisotropy Decay Methods.- 1. Introduction.- 2. Optical Anisotropy Decay.- 3. Examples of Application.- 4. Concluding Remarks.- 5. References.- 3 Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Cell Membranes.- 1. Introduction.- 2. Comparison of Time- and Frequency-Domain Measurements.- 3. Theory of Frequency-Domain Fluorometry.- 4. Intensity Decays of DPH-Labeled Membranes.- 5. Anisotropy Decays of Labeled Membranes.- 6. Time-Resolved Emission Spectra.- 7. Energy Transfer in Membranes.- 8. A 2-GHz Frequency-Domain Fluorometer.- 9. Future Developments.- 10. Summary.- 11. References.- 4 Time-Resolved Fluorescence Depolarization Techniques in Model Membrane Systems: Effect of Sterols and Unsaturations.- 1. Introduction.- 2. Intrinsic Motional Properties of Some Widely Used Fluorescent Probes.- 3. Sterol-Phospholipid Interactions in Model Membranes.- 4. Concluding Remarks.- 5. References.- 5 Fluorescence Polarization to Evaluate the Fluidity of Natural and Reconstituted Membranes.- 1. Introduction.- 2. Methodology.- 3. Current Advancements in the Measurement of Ion-Membrane Interactions Using Fluorescence Polarization.- 4. Critical Evaluation of the Significance of Ion-Membrane Measurements.- 5. Concluding Remarks.- 6. References.- 6 Fluidity of Thyroid Plasma Membranes.- 1. Introduction.- 2. Thyroid Plasma Membranes.- 3. Fluidity of Thyroid Plasma Membranes.- 4. Modulation of the Adenylate Cyclase Activity by Manipulating the Plasma Membrane Composition.- 5. Involvement of Membrane Fluidity on Human Normal and Pathological Thyroid Glands.- 6. References.- 7 Spectroscopic Analysis of the Structure of Bacteriorhodopsin.- 1. Introduction.- 2. Principle of the Fluorescence Energy Transfer Technique.- 3. Three-Dimensional Disposition of the Retinal Chromophore in the Purple Membrane.- 4. In-Plane Location of NBD (7-Chloro-4-Nitrobenzo-2-Oxa-l,3-Diazole) Bound to Lys-41 in the Purple Membrane.- 5. Conformational Prediction of Bacteriorhodopsin Molecule.- 6. References.- 8 Structure and Dynamics of the Liver Microsomal Monoxygenase System.- 1. Introduction.- 2. Membrane Dynamics and Order Studied by Fluorescence.- 3. References.- 9 Fluorescence Studies on Prokaryotic Membranes.- 1. Introduction.- 2. Fluorescent Probes.- 3. Structural Aspects of Bacterial Membranes.- 4. Periplasm.- 5. Incorporation of Exogenous Lipids into Prokaryotic Membranes.- 6. Concluding Remarks.- 7. References.- 10 The Study of Cytoskeletal Protein Interactions by Fluorescence Probe Techniques.- 1. Introduction.- 2. The Cytoskeleton.- 3. Fluorescence Probe Techniques.- 4. Alternative Luminescence Techniques.- 5. Summary and Future Prospects.- 6. References.- 11 Fluorescent Probes for the Acetylcholine Receptor Surface Environments.- 1. Introduction.- 2. An Overview of AchR Properties.- 3. PTSA: A Probe for Measuring AchR-Mediated Ionic Fluxes in the Physiological Time Scale.- 4. Pyrene-1-Sulfonyl Azide (PySA): A Probe for the Study of the AchR-Lipid Interface.- 5. Pyrene Maleimide (PM): The Labeling of a Functionally Relevant Sulfhydryl Group.- 6. State and Organization of the Lipid Bilayer in AchR Membranes.- 7. Summary.- 8. References.- 12 Structural Basis and Physiological Control of Membrane Fluidity in Normal and Tumor Cells.- 1. Introduction.- 2. Quantitative Contribution of Individual Types of Lipid to Membrane Fluidity.- 3. Alterations in Membrane Fluidity in Lymphoid Tumor Cells.- 4. Effects of Dietary Lipids on Membrane Fluidity.- 5. References.- 13 Fusion of Enveloped Viruses with Biological Membranes: Fluorescence Dequenching Studies.- 1. Introduction.- 2. Receptors for Enveloped Viruses.- 3. Interaction of Enveloped Viruses with Receptor-Depleted Cells.- 4. Theoretical Aspects of the Use of Fluorescence Dequenching to Measure Viral Fusion.- 5. Fusion of Enveloped Viruses with Animal Cells and Biological Membranes: Studies with Intact Virions.- 6. Use of Fluorescent Dequenching Methods to Study Fusion of Enveloped Viruses with Biological Membranes Lacking Virus Receptors.- 7. Role of Viral Glycoproteins in the Process of Virus Membrane Fusion: Studies with Reconstituted Viral Envelopes.- 8. Fusion of Enveloped Viruses with Negatively Charged and Neutral Liposomes.- 9. Role of Conformational Changes and Cooperativity of Viral Proteins in Mediating Membrane Fusion.- 10. Conclusions.- 11. References.

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