2015, ISBN: 9783659713279

[ED: Taschenbuch / Paperback], [PU: LAP Lambert Academic Publishing], In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease proc… More...

[ED: Taschenbuch / Paperback], [PU: LAP Lambert Academic Publishing], In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease processes (cancer, diabetes, and drug-sensitivity), their quantitative determination is very important. This book is directed towards the development of methods for the determination of epoxides, ,beta-unsaturated carbonyls and ,beta-dicarbonyls. 1,2-Epoxy-3-butene (EB), methyl vinyl ketone (MVK), and alloxan were used as representative molecules. Following derivatization with N,N-diethyldithiocarbamate (DTC) or 1,2-phenylenediamine (PD), the stable adducts of EIs were analyzed by reversed phase (RP) high-performance liquid chromatography (HPLC) coupled with either UV or fluorescence detection. This facilitated the detection of all three compounds, viz., EB, MVK and alloxan. The detection and reproducibility were accomplished to lower picomole or femtomole levels. Further, this approach was robust and easily adaptable for the analysis of various EIs belonging to the above class of compounds. The methods were suitable for analysis of EI's in biological fluids viz., fetal bovine serum and tissue homogenates of brain, heart, liver and kidney., DE, [SC: 0.00], Neuware, gewerbliches Angebot, H: 220mm, B: 150mm, 128, Selbstabholung und Barzahlung, PayPal, offene Rechnung, Banküberweisung, Internationaler Versand<

ISBN: 9783659713279

[ED: Taschenbuch], [PU: LAP Lambert Academic Publishing], Neuware - In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease proces… More...

[ED: Taschenbuch], [PU: LAP Lambert Academic Publishing], Neuware - In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease processes (cancer, diabetes, and drug-sensitivity), their quantitative determination is very important. This book is directed towards the development of methods for the determination of epoxides, ,beta-unsaturated carbonyls and ,beta-dicarbonyls. 1,2-Epoxy-3-butene (EB), methyl vinyl ketone (MVK), and alloxan were used as representative molecules. Following derivatization with N,N-diethyldithiocarbamate (DTC) or 1,2-phenylenediamine (PD), the stable adducts of EIs were analyzed by reversed phase (RP) high-performance liquid chromatography (HPLC) coupled with either UV or fluorescence detection. This facilitated the detection of all three compounds, viz., EB, MVK and alloxan. The detection and reproducibility were accomplished to lower picomole or femtomole levels. Further, this approach was robust and easily adaptable for the analysis of various EIs belonging to the above class of compounds. The methods were suitable for analysis of EI's in biological fluids viz., fetal bovine serum and tissue homogenates of brain, heart, liver and kidney., DE, [SC: 0.00], Neuware, gewerbliches Angebot, 220x150x8 mm, 128, [GW: 207g], PayPal, Offene Rechnung, Banküberweisung, Sofortüberweisung, Internationaler Versand<

ISBN: 3659713279

[EAN: 9783659713279], Neubuch, Publisher/Verlag: LAP Lambert Academic Publishing | Analyzing Electrophilic Intermediates in Biological Fluids | In view of the reactive properties of Elect… More...

[EAN: 9783659713279], Neubuch, Publisher/Verlag: LAP Lambert Academic Publishing | Analyzing Electrophilic Intermediates in Biological Fluids | In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease processes (cancer, diabetes, and drug-sensitivity), their quantitative determination is very important. This book is directed towards the development of methods for the determination of epoxides, , -unsaturated carbonyls and , -dicarbonyls. 1,2-Epoxy-3-butene (EB), methyl vinyl ketone (MVK), and alloxan were used as representative molecules. Following derivatization with N,N-diethyldithiocarbamate (DTC) or 1,2-phenylenediamine (PD), the stable adducts of EIs were analyzed by reversed phase (RP) high-performance liquid chromatography (HPLC) coupled with either UV or fluorescence detection. This facilitated the detection of all three compounds, viz., EB, MVK and alloxan. The detection and reproducibility were accomplished to lower picomole or femtomole levels. Further, this approach was robust and easily adaptable for the analysis of various EIs belonging to the above class of compounds. The methods were suitable for analysis of EI's in biological fluids viz., fetal bovine serum and tissue homogenates of brain, heart, liver and kidney. | Format: Paperback | Language/Sprache: english | 128 pp<

ISBN: 9783659713279

Paperback, [PU: LAP Lambert Academic Publishing], In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease processes (cancer, diabe… More...

Paperback, [PU: LAP Lambert Academic Publishing], In view of the reactive properties of Electrophilic intermediates (EIs) and their involvement in various disease processes (cancer, diabetes, and drug-sensitivity), their quantitative determination is very important. This book is directed towards the development of methods for the determination of epoxides, α,β-unsaturated carbonyls and α,β-dicarbonyls. 1,2-Epoxy-3-butene (EB), methyl vinyl ketone (MVK), and alloxan were used as representative molecules. Following derivatization with N, N-diethyldithiocarbamate (DTC) or 1,2-phenylenediamine (PD), the stable adducts of EIs were analyzed by reversed phase (RP) high-performance liquid chromatography (HPLC) coupled with either UV or fluorescence detection. This facilitated the detection of all three compounds, viz., EB, MVK and alloxan. The detection and reproducibility were accomplished to lower picomole or femtomole levels. Further, this approach was robust and easily adaptable for the analysis of various EIs belonging to the above class of compounds. The methods were suitable for analysis of EI's in biological fluids viz., fetal bovine serum and tissue homogenates of brain, heart, liver and kidney, Chemistry<

2015, ISBN: 3659713279

Kartoniert / Broschiert, mit Schutzumschlag 11, [PU:LAP LAMBERT Academic Publishing]