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Integrated Genomics by Guy A. Caldwell Paperback | Indigo Chapters
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ISBN: 9780470095027

Integrated Genomics: A Discovery-Based Laboratory Course introduces the excitement of discovery to the basic molecular biology laboratory. Utilizing up-to-date molecular biology protocols… More...

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Integrated Genomics: A Discovery-Based Laboratory Course
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Integrated Genomics - Caldwell, Guy A. Williams, Shelli N. Caldwell, Kim A.
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Caldwell, Guy A. Williams, Shelli N. Caldwell, Kim A.:
Integrated Genomics - First edition

2006

ISBN: 9780470095027

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Integrated Genomics - Caldwell/ Williams/ Kim Caldwell
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Integrated Genomics - Paperback

ISBN: 9780470095027

*Integrated Genomics* - A Discovery-Based Laboratory Course. 1. Auflage / Taschenbuch für 107.99 € / Aus dem Bereich: Bücher, Wissenschaft, Biologie Medien > Bücher nein Buch (kartoniert)… More...

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Integrated Genomics ? A Discovery?Based Laboratory Course - Guy A. Caldwell; Shelli N. Williams; Kim A. Caldwell
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Guy A. Caldwell; Shelli N. Williams; Kim A. Caldwell:
Integrated Genomics ? A Discovery?Based Laboratory Course - Paperback

2006, ISBN: 9780470095027

Buch, Softcover, [PU: John Wiley & Sons Inc], John Wiley & Sons Inc, 2006

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Integrated Genomics
Author:
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ISBN:

9780470095027


Diese spannende, gut nachvollziehbare Einführung in das moderne Gebiet der Genomforschung ist eine gelungene Mischung ais methogologischen Informationen zur Molekularbiologie und Arbeitsanleitungen zur sinnvollen Benutzung online verfügbarer Datenbanken.

Details of the book - Integrated Genomics


EAN (ISBN-13): 9780470095027
ISBN (ISBN-10): 0470095024
Hardcover
Paperback
Publishing year: 2006
Publisher: John Wiley & Sons
225 Pages
Weight: 0,576 kg
Language: eng/Englisch

Book in our database since 2008-06-12T22:22:41+01:00 (London)
Detail page last modified on 2023-04-13T11:36:07+01:00 (London)
ISBN/EAN: 9780470095027

ISBN - alternate spelling:
0-470-09502-4, 978-0-470-09502-7
Alternate spelling and related search-keywords:
Book author: shelli, williams, guy, kim, john caldwell
Book title: integrated genomics discovery based laboratory course

Information from Publisher

Author: Guy Caldwell; Shelli Williams; Kim Caldwell
Title: Integrated Genomics - A Discovery-Based Laboratory Course
Publisher: Wiley; John Wiley & Sons
248 Pages
Publishing year: 2006-07-28
Weight: 0,719 kg
Language: English
79,90 € (DE)
Not available (reason unspecified)
216mm x 279mm x 15mm

BC; Hardcover, Softcover / Biologie/Genetik, Gentechnik; Genetik (nicht-medizinisch); Biowissenschaften; Genetics; Genetik; Genomics; Life Sciences; Genetik; BB

Preface. Author biographies. Acknowledgments. List of figures. 1 Introduction to basic laboratory genetics. 1.1 Transferring and handling C. elegans. 1.2 Introduction to laboratory genetics. 2 Gene expression analysis using transgenic animals. 2.1 Transgenic gene expression analysis in C. elegans: lacZ staining. 2.2 Transgenic gene expression analysis in C. elegans: GFP analysis. 3 Creation and testing of transgenic yeast for use in protein-protein interaction screening. 3.1 Small-scale transformation of S. cerevisiae. 3.2 Transformation of S. cerevisiae to test for non-specific interaction. 3.3 Assaying for protein-protein interaction by reporter gene expression. 4 Yeast two-hybrid screening. 4.1 Protein-protein interaction screening of a C. elegans cDNA library. 4.2 Assaying for protein-protein interaction by reporter gene expression. 5 Isolation and identification of interacting proteins. 5.1 Preparation of electrocompetent E. coli. 5.2 Isolation of DNA from yeast and electroporation of E. coli. 5.3 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. 5.4 Sequencing of two-hybrid library plasmid DNA vectors. 6 Using bioinformatics in modern science. 6.1 DNA sequence chromatogram. 6.2 BLASTing your sequence. 6.3 Evaluating sequence results and choosing an RNAi target. 6.4 Bioinformatics practice questions. 7 Generation of an RNAi vector. 7.1 Small-scale isolation of genomic DNA from C. elegans. 7.2 PCR amplification of target gene sequence from C. elegans genomic DNA. 7.3 Preparations for cloning to generate RNAi vector. 7.3.1 Agarose gel electrophoresis. 7.3.2 Removal of dNTPs from PCR reaction. 7.3.3 Restriction enzyme digestion of PCR product and C. elegans RNAi vector. 7.4 Gel purification of DNA and ligation of vector and PCR-amplified DNA. 7.4.1 Preparative agarose gel electrophoresis. 7.4.2 Gel purification of DNA from agarose gel. 7.4.3 Ligation of vector and PCR-amplified DNA. 7.5 Transformation of ligation reactions. 7.6 PCR screening of transformation colonies. 7.7 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. 7.8 Verifying successful ligation by restriction digestion. 8 RNA-mediated interference by bacterial feeding. 8.1 Preparation of RNAi-feeding bacteria for transformation. 8.2 Media preparation for RNAi feeding. 8.3 Transformation of RNAi-feeding strain HT115(DE3). 8.4 RNA interference by bacterial feeding of C. elegans. 8.5 Analyzing effects of dsRNAi. 8.5.1 Assaying for sterility (Ste) or embryonic lethality (Emb). 8.5.2 Assaying for growth effect. 8.5.3 Assaying for morphological effects. 8.5.4 Assaying for general neuromuscular effects. 8.5.5 Assaying for specific neuronal effects. 8.5.6 Assaying for dauer formation. Appendix I Recombinational cloning. AI.1 Isolation of genomic DNA from C. elegans. AI.2 PCR amplification of target gene sequence from C. elegans genomic DNA. AI.3 Agarose gel electrophoresis and clean-up of PCR reaction. AI.4 Entry vector cloning. AI.5 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. AI.6 Destination vector cloning. AI.7 Small-scale isolation of plasmid DNA from E. coli: the mini-prep. Appendix II Recipes and media preparation. Solution recipes. Media preparation. Appendix III Sterile techniques and worm protocols. Sterile techniques. Worm protocols. Appendix IV Mutant C. elegans phenotypes. Appendix V Vector maps. Subject index.

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